Measurement

Part:BBa_K4628026:Design

Designed by: Qinyao Yan   Group: iGEM23_ZJU-China   (2023-10-11)


CP-GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 440


Design Notes

In our experiment, the construction of the fusion protein can transform the viral load, which is difficult to be directly quantified, into a simple index that can be analyzed by the fluorescence intensity and protein content of the fluorescent protein, so that the experiment will be carried out more smoothly.


Source

We conducted our own cloning transformation in the laboratory.

References